rabbit anti gcnt1 Search Results


rabbit anti gcnt1  (Bioss)
94
Bioss rabbit anti gcnt1
Rabbit Anti Gcnt1, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti gcnt1/product/Bioss
Average 94 stars, based on 1 article reviews
rabbit anti gcnt1 - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
3b5  (Abcam)
95
Abcam 3b5
3b5, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/3b5/product/Abcam
Average 95 stars, based on 1 article reviews
3b5 - by Bioz Stars, 2026-02
95/100 stars
  Buy from Supplier
recombinant anti gne antibody  (Abcam)
95
Abcam recombinant anti gne antibody
(A) Biosynthetic pathway and lectin probes for mucin-type core-I and core-II O-glycans. (B) Flow cytometry analysis of 1E7 cells and their glycoengineered progeny with knockout (KO) of C1GALT1 to truncate O-glycans, KO of <t>GNE</t> to ablate glycan sialylation, and overexpression (OE) of GCNT1 to enhance O-glycan extension; results for epithelial cells that do not <t>express</t> <t>Muc1-GFP</t> (-Muc1-GFP) are included as a control. (C) Western blot showing the relative size of Muc1 polymer in the 1E7 clone and glycoengineered progeny. (D) sWGA lectin blot after extended SDS-PAGE run time showing increased molecular weight of Muc1-GFP in GCNT1-overexpressing 1E7 cells compared to wild-type 1E7 cells. (E) Quantification of glycocalyx thickness in 1E7 cells, their glycoengineered progeny, and galectin-3 overexpressing cells (+Gal3 OE) at the indicated doxycycline induction levels; as indicated, some treatments include 10 µM recombinant galectin-1, 5 µM recombinant galectin-3, or 10 µM of the galectin inhibitor TD139. Results are the mean ± s.d. of at least 13 cells. Statistical significance is given by ns - not significant, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. Results are a representative dataset from 3 independent replicates.
Recombinant Anti Gne Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant anti gne antibody/product/Abcam
Average 95 stars, based on 1 article reviews
recombinant anti gne antibody - by Bioz Stars, 2026-02
95/100 stars
  Buy from Supplier
anti gcnt1 antibody  (Danaher Inc)
86
Danaher Inc anti gcnt1 antibody
(A) Biosynthetic pathway and lectin probes for mucin-type core-I and core-II O-glycans. (B) Flow cytometry analysis of 1E7 cells and their glycoengineered progeny with knockout (KO) of C1GALT1 to truncate O-glycans, KO of <t>GNE</t> to ablate glycan sialylation, and overexpression (OE) of GCNT1 to enhance O-glycan extension; results for epithelial cells that do not <t>express</t> <t>Muc1-GFP</t> (-Muc1-GFP) are included as a control. (C) Western blot showing the relative size of Muc1 polymer in the 1E7 clone and glycoengineered progeny. (D) sWGA lectin blot after extended SDS-PAGE run time showing increased molecular weight of Muc1-GFP in GCNT1-overexpressing 1E7 cells compared to wild-type 1E7 cells. (E) Quantification of glycocalyx thickness in 1E7 cells, their glycoengineered progeny, and galectin-3 overexpressing cells (+Gal3 OE) at the indicated doxycycline induction levels; as indicated, some treatments include 10 µM recombinant galectin-1, 5 µM recombinant galectin-3, or 10 µM of the galectin inhibitor TD139. Results are the mean ± s.d. of at least 13 cells. Statistical significance is given by ns - not significant, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. Results are a representative dataset from 3 independent replicates.
Anti Gcnt1 Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti gcnt1 antibody/product/Danaher Inc
Average 86 stars, based on 1 article reviews
anti gcnt1 antibody - by Bioz Stars, 2026-02
86/100 stars
  Buy from Supplier
rabbit anti gcnt1 center  (RayBiotech)
N/A
Rabbit Anti GCNT1 Center Antibody 400 µl
   Buy from Supplier
rabbit anti human gcnt1 center  (RayBiotech)
N/A
Rabbit Anti Human GCNT1 Center Antibody 400 µl
   Buy from Supplier

Image Search Results


(A) Biosynthetic pathway and lectin probes for mucin-type core-I and core-II O-glycans. (B) Flow cytometry analysis of 1E7 cells and their glycoengineered progeny with knockout (KO) of C1GALT1 to truncate O-glycans, KO of GNE to ablate glycan sialylation, and overexpression (OE) of GCNT1 to enhance O-glycan extension; results for epithelial cells that do not express Muc1-GFP (-Muc1-GFP) are included as a control. (C) Western blot showing the relative size of Muc1 polymer in the 1E7 clone and glycoengineered progeny. (D) sWGA lectin blot after extended SDS-PAGE run time showing increased molecular weight of Muc1-GFP in GCNT1-overexpressing 1E7 cells compared to wild-type 1E7 cells. (E) Quantification of glycocalyx thickness in 1E7 cells, their glycoengineered progeny, and galectin-3 overexpressing cells (+Gal3 OE) at the indicated doxycycline induction levels; as indicated, some treatments include 10 µM recombinant galectin-1, 5 µM recombinant galectin-3, or 10 µM of the galectin inhibitor TD139. Results are the mean ± s.d. of at least 13 cells. Statistical significance is given by ns - not significant, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. Results are a representative dataset from 3 independent replicates.

Journal: bioRxiv

Article Title: Mucins form a nanoscale material barrier against immune cell attack

doi: 10.1101/2022.01.28.478211

Figure Lengend Snippet: (A) Biosynthetic pathway and lectin probes for mucin-type core-I and core-II O-glycans. (B) Flow cytometry analysis of 1E7 cells and their glycoengineered progeny with knockout (KO) of C1GALT1 to truncate O-glycans, KO of GNE to ablate glycan sialylation, and overexpression (OE) of GCNT1 to enhance O-glycan extension; results for epithelial cells that do not express Muc1-GFP (-Muc1-GFP) are included as a control. (C) Western blot showing the relative size of Muc1 polymer in the 1E7 clone and glycoengineered progeny. (D) sWGA lectin blot after extended SDS-PAGE run time showing increased molecular weight of Muc1-GFP in GCNT1-overexpressing 1E7 cells compared to wild-type 1E7 cells. (E) Quantification of glycocalyx thickness in 1E7 cells, their glycoengineered progeny, and galectin-3 overexpressing cells (+Gal3 OE) at the indicated doxycycline induction levels; as indicated, some treatments include 10 µM recombinant galectin-1, 5 µM recombinant galectin-3, or 10 µM of the galectin inhibitor TD139. Results are the mean ± s.d. of at least 13 cells. Statistical significance is given by ns - not significant, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. Results are a representative dataset from 3 independent replicates.

Article Snippet: The following antibodies were used: anti-Human Muc1 Clone HMPV (555925, BD Biosciences), anti-Human Muc1 Janelia Fluor 549 (NBP-2-47883JF549, Novus Biologicals), Recombinant anti-GNE antibody (ab189927, Abcam), anti-GCNT1 antibody (ab102665, Abcam), anti-Galectin-3 N-20 (19280, Santa Cruz), anti-Galectin-1 (ab108389, Abcam), mouse anti-β-Actin Clone C4 (47778, Santa Cruz), anti-ErbB2/HER2 Clone 3B5 (ab16901, Abcam), APC conjugated anti-hErbB2/HER2 (FAB1129A, R&D systems), APC conjugated anti-Human perforin (Clone dG9, 30811, BioLegend), anti-ErbB2/HER2 Clone 3B5 (ab16901, Abcam), Anti-6X His-tag antibody (ab9108, Abcam).

Techniques: Flow Cytometry, Knock-Out, Over Expression, Western Blot, SDS Page, Molecular Weight, Recombinant

(A) NK-92 cell-mediated cytotoxicity of 1E7 cells, their glycoengineered progeny ( C1GALT1 KO, GNE KO, and GCNT1 OE), and 1E7 cells overexpressing galectin-3 (Gal-3 OE) at the indicated doxycycline induction level; NK cell to target cell ratio is 5:1. (B) NK-92 cell-mediated cytotoxicity is inversely proportional to the measured glycocalyx thickness with Pearson correlation coefficient, r = -0.94; dashed line indicates a linear fit to the data. Data presented as the mean and ± s.d. of three independent experiments. Statistical significance is given by ns - not significant, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. Results are a representative dataset from 3 independent replicates.

Journal: bioRxiv

Article Title: Mucins form a nanoscale material barrier against immune cell attack

doi: 10.1101/2022.01.28.478211

Figure Lengend Snippet: (A) NK-92 cell-mediated cytotoxicity of 1E7 cells, their glycoengineered progeny ( C1GALT1 KO, GNE KO, and GCNT1 OE), and 1E7 cells overexpressing galectin-3 (Gal-3 OE) at the indicated doxycycline induction level; NK cell to target cell ratio is 5:1. (B) NK-92 cell-mediated cytotoxicity is inversely proportional to the measured glycocalyx thickness with Pearson correlation coefficient, r = -0.94; dashed line indicates a linear fit to the data. Data presented as the mean and ± s.d. of three independent experiments. Statistical significance is given by ns - not significant, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. Results are a representative dataset from 3 independent replicates.

Article Snippet: The following antibodies were used: anti-Human Muc1 Clone HMPV (555925, BD Biosciences), anti-Human Muc1 Janelia Fluor 549 (NBP-2-47883JF549, Novus Biologicals), Recombinant anti-GNE antibody (ab189927, Abcam), anti-GCNT1 antibody (ab102665, Abcam), anti-Galectin-3 N-20 (19280, Santa Cruz), anti-Galectin-1 (ab108389, Abcam), mouse anti-β-Actin Clone C4 (47778, Santa Cruz), anti-ErbB2/HER2 Clone 3B5 (ab16901, Abcam), APC conjugated anti-hErbB2/HER2 (FAB1129A, R&D systems), APC conjugated anti-Human perforin (Clone dG9, 30811, BioLegend), anti-ErbB2/HER2 Clone 3B5 (ab16901, Abcam), Anti-6X His-tag antibody (ab9108, Abcam).

Techniques: